Journal: Biomaterials Research

Article Title: Bioactive self-healing hydrogel based on tannic acid modified gold nano-crosslinker as an injectable brain implant for treating Parkinson’s disease

doi: 10.1186/s40824-023-00347-0

Figure Lengend Snippet: Biocompatibility evaluation of the hydrogels for treating PD rats. A In vivo immunofluorescent images of CD86 positive (CD86+) M1 macrophages (red) and CD163 positive (CD163+) M2 macrophages (green) for the explanted tissue after implantation in the brain for 14 days. Quantification for ( B ) the fluorescent intensity of M1 and M2 macrophages and ( C ) the ratios of M2/M1 in vivo. * p < 0.05, ** p < 0.01, and **** p < 0.0001 between the indicated groups

Article Snippet: The primary antibodies used in this study involved Anti Iba1 (Rabbit, for immunocytochemistry, 019–19,741, 1:500, Wako Pure Chemical Industries, Ltd., Japan), ABclonal rabbit polyclonal anti-CD86 antibody (A1199, 1:200, ABclonal Inc., USA), ABclonal rabbit polyclonal anti-CD163 antibody (A8383, 1:200, ABclonal Inc., USA), recombinant anti- tyrosine hydroxylase (TH) antibody (EP1533Y/ab75875, 1:50, Abcam, UK), and mouse monoclonal anti-GFAP antibody (14–9892-82, 1:100, Invitrogen, USA).

Techniques: In Vivo

Journal: Frontiers in Cardiovascular Medicine

Article Title: PACAP regulates VPAC1 expression, inflammatory processes and lipid homeostasis in M1- and M2-macrophages

doi: 10.3389/fcvm.2023.1264901

Figure Lengend Snippet: List of used antibodies.

Article Snippet: Rabbit anti-mouse CD86 , Orb10351 , Biorbyt, Cambridge, UK , 1:50.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: PACAP regulates VPAC1 expression, inflammatory processes and lipid homeostasis in M1- and M2-macrophages

doi: 10.3389/fcvm.2023.1264901

Figure Lengend Snippet: Effects of PACAP deficiency on the distribution and amount of different MΦ subtypes of atherosclerotic plaques in the aortic arch. Immunohistomorphometric analyses of atherosclerotic lesions in ApoE −/− and PACAP −/− /ApoE −/− mice after SC or CED. Analyses of VPAC1 ( A ), CD86 ( B ), CD68 ( C ), and CD163 ( D ) on cryosections of the aortic arch and representative photomicrographs ( E–T ) are shown. The graph bar is given as the mean. Black dots represent single measurement. ( n = 3–8). * p ≤ 0.05 vs. ApoE −/− after 30 weeks of SC; # p ≤ 0.05; ### p ≤ 0.001 vs. ApoE −/− after 20 weeks of CED. SC, standard diet; CED, cholesterol-enriched diet; n, number of experimental animals; Black arrows, positive reaction; asterisks, vessel lumen. Scale bar = 10 μm; Overview screen scale bar = 100 µm.

Article Snippet: Rabbit anti-mouse CD86 , Orb10351 , Biorbyt, Cambridge, UK , 1:50.

Techniques: