Journal: ACS Omega

Article Title: M1 Macrophages Enhance Survival and Invasion of Oral Squamous Cell Carcinoma by Inducing GDF15-Mediated ErbB2 Phosphorylation

doi: 10.1021/acsomega.2c00571

Figure Lengend Snippet: Primary Antibodies and Dilution Ratio for Western Blotting

Article Snippet: rabbit anti-CD86 , Cell Signaling Technology, Danvers, MA , 1:1000.

Techniques: Western Blot

Journal: Biomaterials Research

Article Title: Bioactive self-healing hydrogel based on tannic acid modified gold nano-crosslinker as an injectable brain implant for treating Parkinson’s disease

doi: 10.1186/s40824-023-00347-0

Figure Lengend Snippet: Biocompatibility evaluation of the hydrogels for treating PD rats. A In vivo immunofluorescent images of CD86 positive (CD86+) M1 macrophages (red) and CD163 positive (CD163+) M2 macrophages (green) for the explanted tissue after implantation in the brain for 14 days. Quantification for ( B ) the fluorescent intensity of M1 and M2 macrophages and ( C ) the ratios of M2/M1 in vivo. * p < 0.05, ** p < 0.01, and **** p < 0.0001 between the indicated groups

Article Snippet: The primary antibodies used in this study involved Anti Iba1 (Rabbit, for immunocytochemistry, 019–19,741, 1:500, Wako Pure Chemical Industries, Ltd., Japan), ABclonal rabbit polyclonal anti-CD86 antibody (A1199, 1:200, ABclonal Inc., USA), ABclonal rabbit polyclonal anti-CD163 antibody (A8383, 1:200, ABclonal Inc., USA), recombinant anti- tyrosine hydroxylase (TH) antibody (EP1533Y/ab75875, 1:50, Abcam, UK), and mouse monoclonal anti-GFAP antibody (14–9892-82, 1:100, Invitrogen, USA).

Techniques: In Vivo

Journal: Frontiers in Cell and Developmental Biology

Article Title: Deciphering perivascular macrophages and microglia in the retinal ganglion cell layers

doi: 10.3389/fcell.2024.1368021

Figure Lengend Snippet: Representative histological images for minor populations of the BAM with CD86 expression around the optic nerve head. (A) Representative histological images of the CD86+/CX3CR1 cells around the optic nerve head and proximal retinal vein. Arrowheads indicate CD86+/CX3CR1+ cells. (B) Quantification of the total number of CD86 cells in the retina except the area within two disc distance ( n = 6/4/6/8). (C) Schematic design of the transgenic mouse and experiment time line for visualizing the microglia-specific CD86 RFP expression. (D) Representative images of a CD86i△Mg RFP mouse on each layer compared with CD86 antibody-stained images showing that the soma of the transgenic mice in the all layers have CD86 expression. Arrowheads indicate CD86+/CX3CR1+ cells. (E) Representative mosaic images show that there is CD86 expression on the microglial processes in the proximal retinal vein. The data are presented as the mean ± SD. Scale bar: 100 μm.

Article Snippet: These processed samples (flat-mounted and sliced) were blocked with 5% normal goat serum (NS02L-1ML, Sigma-Aldrich) or bovine serum albumin (BSA, A7030-10G, Sigma-Aldrich) in PBST (0.3% Triton X-100 in PBS) and incubated overnight at 4°C with the following primary antibodies (1:200): anti-CD31 (PECAM, rat, #553708, BD Biosciences, Franklin Lakes, New Jersey, United States, hamster, 2H8 clone, MA3105, Thermo Fisher), anti-Iba1 (rabbit, #PA5-27436, Thermo Fisher Scientific, Waltham, Massachusetts, United States), anti-galectin-3 (rabbit, #ab209344, Abcam Inc., Cambridge, United Kingdom), anti-LYVE-1 (rabbit, #ab218535, Abcam Inc.), anti-CD86 (rabbit, #ab242142, Abcam Inc.), and anti-CD44 (rabbit, #ab243894, Abcam Inc.).

Techniques: Expressing, Transgenic Assay, Staining